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Structured Review

Corning Life Sciences cs10 cell stack
Cs10 Cell Stack, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+stack/t+flasks/pm40430080-208-16-21
Average 90 stars, based on 1 article reviews
cs10 cell stack - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Transfection:

Article Title: Identification of an adeno-associated virus binding epitope for AVB sepharose affinity resin
Article Snippet: Purified vector preparations AAV2/8.CMV.ffluciferase.SV40 AAV2/rh.64R1.CMV.PI.EGFP.WPRE.bGH, AAV2/hu.37.TBG.EGFP.bGH, and AAV2/rh.10.CMV.PI.Cre.RBG were produced and titrated by Penn Vector Core as previously described. .. Briefly, one cell stack (Corning, NY) of HEK293 cells was transfected with triple-plasmid cocktail by polyethylenimine when the cell confluency reached around 85%. .. Culture supernatant was harvested 5 days posttransfection and digested with turbonuclease (Accelagen, CA).

Article Title: Autophagy-independent lysosomal targeting regulated by ULK1/2-FIP200 and ATG9
Article Snippet: Viral Packaging For large scale production of the lentiviral gRNA library, 210 × 10 6 HEK293T cells were plated in cell stack (Corning) 24hrs prior to transfection. .. Viral Packaging For large scale production of the lentiviral gRNA library, 210 × 10 6 HEK293T cells were plated in cell stack (Corning) 24hrs prior to transfection. .. For each cell stack, cells were transfected using 510.3 μL of TransIT reagent diluted 18.4 mL of OPTI-MEM that was combined with 75.6 μg of the gRNA library and 94.5 μg of the Cellecta packaging mix (containing the psPAX2 and pMD2 plasmids that encode Gag/Pol and VSV-G respectively).

Article Title: Autophagy-independent lysosomal targeting regulated by ULK1/2-FIP200 and ATG9
Article Snippet: For large scale production of the lentiviral gRNA library, 210 × 10 6 HEK293T cells were plated in cell stack (Corning) 24hrs prior to transfection. .. For large scale production of the lentiviral gRNA library, 210 × 10 6 HEK293T cells were plated in cell stack (Corning) 24hrs prior to transfection. ..

Article Title: Scalable purification method for AAVRH10
Article Snippet: .. Briefly, one cell stack (Corning, N.Y.) of HEK293 cells was transfected with triple-plasmid cocktail by Polyethylenimine (PEI) when the cell confluency reached around 85%. .. Culture supernatant was harvested 5 days post transfection and digested with Turbonuclease (Accelagen, CA).

Cell Culture:

Article Title: Efficacious and Safe Tissue-Selective Controlled Gene Therapy Approaches for the Cornea
Article Snippet: .. One Cell Stack (Corning Inc., Corning, NY, USA) with approximately 1×10 9 HEK 293 cells was cultured in Dulbecco's Modified Eagle's Medium (Hyclone Laboratories, Inc. Logan UT, USA), supplemented with 5% fetal bovine serum and antibiotics. ..

Modification:

Article Title: Efficacious and Safe Tissue-Selective Controlled Gene Therapy Approaches for the Cornea
Article Snippet: .. One Cell Stack (Corning Inc., Corning, NY, USA) with approximately 1×10 9 HEK 293 cells was cultured in Dulbecco's Modified Eagle's Medium (Hyclone Laboratories, Inc. Logan UT, USA), supplemented with 5% fetal bovine serum and antibiotics. ..

Article Title: Methods and compositions for treating metastatic breast cancer and other cancers in the brain
Article Snippet: Sanger sequencing (GeneWiz) was used to confirm complementarity of pAAV.CB7.CI.201IA.rBG [SEQ ID NO: 5 (SEQ ID NO: 6 corresponds to encoded 201IA sequence)], pAAV.CB7.CI.201IA(I253A).rBG [SEQ ID NO: 7 (encoding SEQ ID NO:8)] and pAAV.CB7.CI.201IA(H435A).rBG [SEQ ID NO: 9 (encoding SEQ ID NO:10] on either side of the desired mutation. .. B. IA Expression in HEK293 Cells and Purification by Protein A 3×108 HEK293 cells (293 cells) were seeded in a 10-stack Cell STACK® (Corning) in DuLbecco's Modified Eagle's Medium (DMEM, Corning CellGro) supplemented with 10% FBS and 1% penicillin/streptomycin (DMEM complete) and incubated at 37° C. 5% CO2 for 48 hours. .. 1 mg of pAAV.CB7.CI.201IA.rBG or pAAV.CB7.CI.201IA(I253A).rBG in TE buffer (Qiagen) was diluted in 42 mL room-temperature antibiotic and serum-free DMEM.

Genome Wide:

Article Title: Genome-wide CRISPR screen for PARKIN regulators reveals transcriptional repression as a determinant of mitophagy
Article Snippet: Selection was optimized by determining the puromycin dose required to achieve >95% cell killing in 96 h. Cell viability was measured for a six-point dose-response ranging from 0 to 10 μg/mL puromycin using the Incucyte Live Cell Analysis System (Essen BioScience). .. For the genome-wide screen, cells were seeded at 167.5 million cells per cell stack (Corning) in media containing 5 μg/mL polybrene and lentivirus at an MOI of 0.5. ..

Article Title: Functional CRISPR screening identifies the ufmylation pathway as a regulator of SQSTM1/p62
Article Snippet: Cell viability was measured with a Cell Titer Glo assay (Promega, Madison, WI) for a 6-point dose response ranging from 0 to 5 mg puromycin. .. For the genome-wide screen, 67 million cells were seeded at 33.5 million cells per cell stack (Corning). ..

Expressing:

Article Title: Methods and compositions for treating metastatic breast cancer and other cancers in the brain
Article Snippet: Sanger sequencing (GeneWiz) was used to confirm complementarity of pAAV.CB7.CI.201IA.rBG [SEQ ID NO: 5 (SEQ ID NO: 6 corresponds to encoded 201IA sequence)], pAAV.CB7.CI.201IA(I253A).rBG [SEQ ID NO: 7 (encoding SEQ ID NO:8)] and pAAV.CB7.CI.201IA(H435A).rBG [SEQ ID NO: 9 (encoding SEQ ID NO:10] on either side of the desired mutation. .. B. IA Expression in HEK293 Cells and Purification by Protein A 3×108 HEK293 cells (293 cells) were seeded in a 10-stack Cell STACK® (Corning) in DuLbecco's Modified Eagle's Medium (DMEM, Corning CellGro) supplemented with 10% FBS and 1% penicillin/streptomycin (DMEM complete) and incubated at 37° C. 5% CO2 for 48 hours. .. 1 mg of pAAV.CB7.CI.201IA.rBG or pAAV.CB7.CI.201IA(I253A).rBG in TE buffer (Qiagen) was diluted in 42 mL room-temperature antibiotic and serum-free DMEM.

Purification:

Article Title: Methods and compositions for treating metastatic breast cancer and other cancers in the brain
Article Snippet: Sanger sequencing (GeneWiz) was used to confirm complementarity of pAAV.CB7.CI.201IA.rBG [SEQ ID NO: 5 (SEQ ID NO: 6 corresponds to encoded 201IA sequence)], pAAV.CB7.CI.201IA(I253A).rBG [SEQ ID NO: 7 (encoding SEQ ID NO:8)] and pAAV.CB7.CI.201IA(H435A).rBG [SEQ ID NO: 9 (encoding SEQ ID NO:10] on either side of the desired mutation. .. B. IA Expression in HEK293 Cells and Purification by Protein A 3×108 HEK293 cells (293 cells) were seeded in a 10-stack Cell STACK® (Corning) in DuLbecco's Modified Eagle's Medium (DMEM, Corning CellGro) supplemented with 10% FBS and 1% penicillin/streptomycin (DMEM complete) and incubated at 37° C. 5% CO2 for 48 hours. .. 1 mg of pAAV.CB7.CI.201IA.rBG or pAAV.CB7.CI.201IA(I253A).rBG in TE buffer (Qiagen) was diluted in 42 mL room-temperature antibiotic and serum-free DMEM.

Incubation:

Article Title: Methods and compositions for treating metastatic breast cancer and other cancers in the brain
Article Snippet: Sanger sequencing (GeneWiz) was used to confirm complementarity of pAAV.CB7.CI.201IA.rBG [SEQ ID NO: 5 (SEQ ID NO: 6 corresponds to encoded 201IA sequence)], pAAV.CB7.CI.201IA(I253A).rBG [SEQ ID NO: 7 (encoding SEQ ID NO:8)] and pAAV.CB7.CI.201IA(H435A).rBG [SEQ ID NO: 9 (encoding SEQ ID NO:10] on either side of the desired mutation. .. B. IA Expression in HEK293 Cells and Purification by Protein A 3×108 HEK293 cells (293 cells) were seeded in a 10-stack Cell STACK® (Corning) in DuLbecco's Modified Eagle's Medium (DMEM, Corning CellGro) supplemented with 10% FBS and 1% penicillin/streptomycin (DMEM complete) and incubated at 37° C. 5% CO2 for 48 hours. .. 1 mg of pAAV.CB7.CI.201IA.rBG or pAAV.CB7.CI.201IA(I253A).rBG in TE buffer (Qiagen) was diluted in 42 mL room-temperature antibiotic and serum-free DMEM.



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